Mogi Y, Kogawa K, Takayama T, Yoshizaki N, Bannai K, Muramatsu H, Koike K, Kohgo Y, Watanabe N, Niitsu Y
Department of Internal Medicine (Section 4), Sapporo Medical College.
Jpn J Cancer Res. 1991 Feb;82(2):192-8. doi: 10.1111/j.1349-7006.1991.tb01828.x.
We established five clones (ML-01, ML-02, MH-01, MH-02, MH-03) from murine 3-methylcholanthrene-induced fibrosarcoma A (Meth A), and investigated their experimental metastatic potentials in relation to their platelet-aggregating activities. A clone with a high metastatic potential (MH-02) showed a characteristic biphasic pattern of platelet aggregation, of which the first peak was not present in the aggregation patterns of the clone with low metastatic potential (ML-01). The first peak was eliminated by treatment of the cells with apyrase, indicating that adenosine diphosphate (ADP) was the causative substance of this particular peak. The metastatic potential of clones correlated well with the ADP concentration of the culture media. These results suggest that the increased ADP production and consequential enhancement of platelet-aggregating activity are closely related to the increment of pulmonary metastatic potential of MH-02 clone.
我们从鼠源3-甲基胆蒽诱导的纤维肉瘤A（Meth A）中建立了五个克隆（ML-01、ML-02、MH-01、MH-02、MH-03），并研究了它们与血小板聚集活性相关的实验转移潜能。具有高转移潜能的克隆（MH-02）呈现出特征性的双相血小板聚集模式，而低转移潜能的克隆（ML-01）的聚集模式中不存在第一个峰值。用腺苷三磷酸双磷酸酶处理细胞可消除第一个峰值，表明二磷酸腺苷（ADP）是这个特定峰值的致病物质。克隆的转移潜能与培养基中的ADP浓度密切相关。这些结果表明，ADP产生增加以及随之而来的血小板聚集活性增强与MH-02克隆肺转移潜能的增加密切相关。
