Balashev V N, Polosova R G
Antibiotiki. 1981 May;26(5):374-8.
The effect of tetracycline hydrochloride on the levels of total protein and separate protein fractions, such as albumins and alpha 1-, alpha 2-, beta- and gamma-globulins in the lymph and blood serum of rabbits was studied. The antibiotic was administered intramuscularly in a single dose of 25 mg/kg or repeatedly for 7 and 20 days. The lymph was collected from the thoracic duct 1, 1.5, 2, 2.5, 3, 3.5, 4 and 24 hours after the antibiotic administration in a single dose. When the antibiotic was used repeatedly, the lymph was collected 24 hours after the last injection. The levels of the total protein were determined colorimetrically and those of the protein fractions were determined turbidimetrically. The results indicated that under the experimental conditions tetracycline induced a marked decrease in the contents of both the total protein and the protein fractions in the lymph and blood serum of the animals. This phenomenon was observed in all periods. Comparative analysis of the data on the lymph and blood serum specimens collected from the same experimental animal revealed a difference in the albumin-globulin ratio, which was higher in the lymph and lower in the blood serum compared to that in the intact animals. This is indicative of the tetracycline capacity for decreasing mainly the levels of the globulin fraction in the lymph and the albumin fraction in the blood serum. The decrease in the level of the gamma-globulin fraction in the lymph and blood serum may be attributed to inhibition of the immune system function due to antibiotic therapy. Since maintenance of the albumin level is one of the liver important functions, the changes in the protein composition of the lymph provide an additional information on the effect of tetracycline on this function of the liver.
研究了盐酸四环素对兔淋巴液和血清中总蛋白及各蛋白组分（如白蛋白、α1 -、α2 -、β -和γ -球蛋白）水平的影响。抗生素以25mg/kg的单次剂量或连续7天和20天肌肉注射给药。单次给药后1、1.5、2、2.5、3、3.5、4和24小时从胸导管收集淋巴液。多次使用抗生素时，在最后一次注射后24小时收集淋巴液。总蛋白水平采用比色法测定，蛋白组分水平采用比浊法测定。结果表明，在实验条件下，四环素导致动物淋巴液和血清中总蛋白及蛋白组分含量显著降低。在所有时间段均观察到这一现象。对同一实验动物采集的淋巴液和血清标本数据的比较分析显示，白蛋白与球蛋白的比例存在差异，与正常动物相比，淋巴液中的该比例较高，血清中的较低。这表明四环素主要降低淋巴液中球蛋白组分水平以及血清中白蛋白组分水平。淋巴液和血清中γ -球蛋白组分水平的降低可能归因于抗生素治疗对免疫系统功能的抑制。由于维持白蛋白水平是肝脏的重要功能之一，淋巴液蛋白质组成的变化为四环素对肝脏这一功能的影响提供了额外信息。
