Reztsova V V
Antibiotiki. 1981 May;26(5):391-3.
Efficiency and duration of suppression of the synthesis of low-polymer (4S) and ribosomal (18S and 28S) fractions of total RNA due to exposure of the cells of Ehrlich's carcinoma and Zajdela's hepatoma to olivomycin were studied. The tumors differed in their sensitivity to the antibiotic. The efficiency of the rRNA synthesis inhibition by olivomycin on its addition to the cells of Ehrlich's carcinoma and Zajdela's hepatoma in vitro was of the same order. Study on the kinetics of rRNA synthesis after addition of olivomycin showed that the maximum inhibition of rRNA synthesis in the cells of Ehrlich's carcinoma was achieved 1 hour after the drug addition. After 2 hours the inhibition level returned to normal. The most efficient inhibition of rRNA synthesis in the cells of Zajdela's hepatoma was observed 2 hours after addition of the drug. The inhibition level in this case did not return to normal after 24 hours. It is concluded that tumor sensitivity to olivomycin is determined by the duration of the affection of the DNA template activity during the synthesis of RNA.
研究了埃利希氏癌和扎伊德氏肝癌细胞暴露于橄榄霉素后，总RNA中低聚物（4S）和核糖体（18S和28S）组分合成受抑制的效率和持续时间。这两种肿瘤对该抗生素的敏感性不同。在体外将橄榄霉素添加到埃利希氏癌细胞和扎伊德氏肝癌细胞中时，其抑制rRNA合成的效率处于同一水平。对添加橄榄霉素后rRNA合成动力学的研究表明，在添加药物1小时后，埃利希氏癌细胞中的rRNA合成受到最大抑制。2小时后，抑制水平恢复正常。在添加药物2小时后，观察到扎伊德氏肝癌细胞中的rRNA合成受到最有效的抑制。在这种情况下，24小时后抑制水平未恢复正常。得出的结论是，肿瘤对橄榄霉素的敏感性取决于RNA合成过程中DNA模板活性受影响的持续时间。
