Barrett S G, Hansen K S, Bainton D F
Br J Haematol. 1981 Jul;48(3):491-500. doi: 10.1111/j.1365-2141.1981.tb02741.x.
Receptors for IgG and complement are present on the plasma membrane of functionally mature blood neutrophils. Neutrophilic precursors lack these membrane receptors, which may account for their diminished phagocytic capability. In this study, myeloid precursors from human bone marrow were examined for the presence and activity of membrane receptors for IgG and activated complement. Morphologic identification by light microscopy of neutrophilic cells are made by rosetting techniques and chloroacetate esterase cytochemical staining and at the ultrastructural level by peroxidase reactivity. This study showed the Fc receptor was the only membrane marker on the myeloblasts and that the number of cells with the receptor increased with maturation. Functional investigation of the Fc receptor indicated that its presence on early myeloid cells was insufficient to induce IgG-mediated ingestion. Phagocytosis via the Fc receptor was not acquired until the metamyelocyte stage. Complement receptors, C3d and C3b, appeared later than the Fc receptor. The C3d receptor, detected first on the promyelocyte, remained relatively constant throughout maturation, whereas the C3b receptor acquired acquired at the myelocyte stage increased with maturation. These data provide evidence that distinct membrane changes occur during normal myeloid cell maturation and that the presence of these membrane receptors is not sufficient for Fc-mediated ingestion to occur.
功能成熟的血液中性粒细胞质膜上存在IgG和补体受体。嗜中性粒细胞前体缺乏这些膜受体，这可能是它们吞噬能力减弱的原因。在本研究中，检测了来自人类骨髓的髓系前体中IgG和活化补体膜受体的存在及活性。通过玫瑰花结技术和氯乙酸酯酶细胞化学染色，利用光学显微镜对嗜中性细胞进行形态学鉴定，并在超微结构水平通过过氧化物酶反应性进行鉴定。本研究表明，Fc受体是成髓细胞上唯一的膜标记物，且带有该受体的细胞数量随成熟度增加。对Fc受体的功能研究表明，其在早期髓系细胞上的存在不足以诱导IgG介导的摄取。直到晚幼粒细胞阶段才获得通过Fc受体的吞噬作用。补体受体C3d和C3b比Fc受体出现得晚。首先在早幼粒细胞上检测到的C3d受体在整个成熟过程中保持相对恒定，而在中幼粒细胞阶段获得的C3b受体则随成熟度增加。这些数据证明，正常髓系细胞成熟过程中会发生明显的膜变化，且这些膜受体的存在不足以引发Fc介导的摄取。
