Sauer R T, Pan J, Hopper P, Hehir K, Brown J, Poteete A R
Biochemistry. 1981 Jun 9;20(12):3591-8. doi: 10.1021/bi00515a044.
The amino acid sequence of the Salmonella phage P2 repressor and the DNA sequence of its gene c2 have been determined. Sequential Edman degradations on intact P22 repressor and repressor peptides generated by proteolytic and chemical cleavages have been overlapped to give approximately 97% of the complete protein sequence. Additionally, the nucleotide sequence of the P22 c2 repressor gene has been determined by DNA sequencing techniques. The DNA sequence and partial protein sequence are collinear and together define the complete amino acid sequence of P22 repressor. The repressor is a single-chain 216 amino acid polypeptide. Basic residues in the sequence tend to be clustered, and residues 9-20 are highly basic, containing five arginyl and three lysyl residues. The carboxyl-terminal 133 amino acids of the c2 repressor are homologous to the carboxyl-terminal sequence of the coliphage lambda cI repressor. The amino-terminal sequences of these two repressors show little similarity.
沙门氏菌噬菌体P2阻遏物的氨基酸序列及其c2基因的DNA序列已被确定。对完整的P22阻遏物以及通过蛋白水解和化学裂解产生的阻遏物肽段进行的连续埃德曼降解已进行重叠，以得出约97%的完整蛋白质序列。此外，P22 c2阻遏物基因的核苷酸序列已通过DNA测序技术确定。DNA序列和部分蛋白质序列是共线的，共同确定了P22阻遏物的完整氨基酸序列。该阻遏物是一条由216个氨基酸组成的单链多肽。序列中的碱性残基倾向于聚集，第9至20位残基高度碱性，包含五个精氨酰基和三个赖氨酰基残基。c2阻遏物的羧基末端133个氨基酸与大肠杆菌噬菌体λ cI阻遏物的羧基末端序列同源。这两种阻遏物的氨基末端序列几乎没有相似性。
