Koppel D E, Sheetz M P, Schindler M
Biophys J. 1980 Apr;30(1):187-92. doi: 10.1016/S0006-3495(80)85087-9.
A new approach is described for the analysis of lateral diffusion in biological membranes. It is shown that a suitably defined first moment of the concentration distribution on a spherical surface decays as a single exponential with a relaxation rate proportional to the diffusion coefficient and inversely proportional to the square of the radius of the sphere. The approach is illustrated with an example of fluorescence redistribution after photobleaching of membrane proteins in a spectrin-deficient spherocytic mouse erythrocyte membrane.
本文描述了一种分析生物膜中横向扩散的新方法。结果表明,球面上浓度分布的适当定义的一阶矩以单指数形式衰减,其弛豫速率与扩散系数成正比,与球半径的平方成反比。通过对血影蛋白缺陷型球形小鼠红细胞膜中膜蛋白光漂白后荧光重新分布的例子来说明该方法。