Koppel D E
Biophys J. 1985 Mar;47(3):337-47. doi: 10.1016/S0006-3495(85)83924-2.
The normal-mode analysis of fluorescence redistribution after photobleaching, introduced for the characterization of lateral diffusion on spherical membrane surfaces, has been generalized and extended to other surface geometries. Theoretical expressions are derived for the characteristic values and orthogonal characteristic functions of the diffusion equations for cylindrical surfaces, ellipsoids of revolution and dimpled discoidal surfaces. On the basis of these results, a simple analytical function is proposed as an empirical solution for the analysis of photobleaching data on a variety of discoidal surfaces. Special experimental and computational methods for determining the surface-diffusion coefficient are described, and demonstrated with data for lipid diffusion in erythrocyte membranes.
用于表征球形膜表面横向扩散的光漂白后荧光重新分布的正常模式分析已被推广并扩展到其他表面几何形状。推导了圆柱面、旋转椭球体和带凹痕的盘状表面扩散方程的特征值和正交特征函数的理论表达式。基于这些结果，提出了一个简单的解析函数作为经验解，用于分析各种盘状表面上的光漂白数据。描述了确定表面扩散系数的特殊实验和计算方法，并以红细胞膜中脂质扩散的数据进行了演示。
