固定在聚乙烯醇薄膜中的细菌视紫红质的光循环。

The photocycle of bacteriorhodopsin immobilized in poly(vinyl alcohol) film.

作者信息

Bryl K, Varo G, Drabent R

机构信息

Department of Physics and Biophysics, University of Agriculture and Technology, Olsztyn, Poland.

出版信息

FEBS Lett. 1991 Jul 8;285(1):66-70. doi: 10.1016/0014-5793(91)80726-j.

DOI:10.1016/0014-5793(91)80726-j

PMID:2065783

Abstract

The kinetics of photoelectric and optical signals were measured on samples containing oriented purple membranes immobilized in a poly(vinyl alcohol) film and on purple membranes introduced into a PVA-H2O mixture. The bacteriorhodopsin photocycle in the PVA-H2O mixture was complete. The only observed changes were the slowing down of the optical and electrical signals in relation to the M412-O640 and O640-bRall-trans steps. In the PVA film the O640 intermediate disappeared and a negative photoelectric signal appeared.

摘要

在含有固定于聚乙烯醇薄膜中的定向紫膜的样品以及引入聚乙烯醇-水混合物中的紫膜上，测量了光电信号和光信号的动力学。紫膜视紫红质在聚乙烯醇-水混合物中的光循环是完整的。唯一观察到的变化是相对于M412-O640和O640-bR全反式步骤，光信号和电信号减慢。在聚乙烯醇薄膜中，O640中间体消失，出现了负光电信号。