大囊泡与表面的粘附分析。
Analysis of adhesion of large vesicles to surfaces.
作者信息
Evans E A
出版信息
Biophys J. 1980 Sep;31(3):425-31. doi: 10.1016/S0006-3495(80)85069-7.
An experimental procedure that can be used to measure the interfacial free energy density for the adhesion of membranes of large vesicles to other surfaces is outlined and analyzed. The approach can be used for both large phospholipid bilayer vesicles and red blood cells when the membrane force resultants are dominated by isotropic tension. The large vesicle or red cell is aspirated by a micropipet with sufficient suction pressure to form a spherical segment outside the pipet. The vesicle is then brought into close proximity of the surface to be tested and, the suction pressure reduced to permit adhesion, and the new equilibrium configuration is established. The mechanical analysis of the equilibrium shape provides the interfacial free energy density for the surface affinity. With this approach, the measurable range of membrane surface affinity is 10(-4)-3 erg/cm2 for large phospholipid bilayer vesicles and 10(-2)-10 erg/cm2 for red blood cells.
概述并分析了一种可用于测量大囊泡膜与其他表面粘附的界面自由能密度的实验方法。当膜合力由各向同性张力主导时，该方法可用于大的磷脂双层囊泡和红细胞。用微量移液器以足够的抽吸压力抽吸大囊泡或红细胞，以在移液器外部形成球形段。然后将囊泡靠近待测试的表面，降低抽吸压力以允许粘附，并建立新的平衡构型。对平衡形状的力学分析提供了表面亲和力的界面自由能密度。通过这种方法，大磷脂双层囊泡的膜表面亲和力可测量范围为10^(-4)-3尔格/平方厘米，红细胞为10^(-2)-10尔格/平方厘米。
Zotero 插件