Korpi E R, Oja S S
Brain Res. 1981 Jul 6;216(1):219-23. doi: 10.1016/0006-8993(81)91293-2.
The distribution of the specific radioactivity and the incorporation into protein of [3H]tryptophan and [3H]valine at varying layers from surface to centre were measured in incubated slices of cerebral cortices from infant and adult rats. Specific radioactivity in free amino acids was in both age groups highest in the intact surface layer. Incorporation of tryptophan into protein was even in slices from adult rats but much less than the average in the surface layers in slices from infant rats. Incorporation of valine exhibited similar heterogeneity in both age groups. The results suggest in brain slice preparations a zonal compartmentation of amino acid and protein metabolism which varies for different amino acids.
在新生和成年大鼠大脑皮层的孵育切片中，测量了从表面到中心不同层面的[3H]色氨酸和[3H]缬氨酸的比放射性分布以及它们掺入蛋白质的情况。游离氨基酸中的比放射性在两个年龄组中均在完整的表层最高。色氨酸掺入蛋白质的情况在成年大鼠的切片中是均匀的，但远低于新生大鼠切片表层的平均值。缬氨酸的掺入在两个年龄组中表现出相似的异质性。结果表明，在脑切片制备中，氨基酸和蛋白质代谢存在区域分隔，且不同氨基酸的情况有所不同。
