Pavlík A, Jakoubek B
Brain Res. 1978 Oct 6;154(1):95-104. doi: 10.1016/0006-8993(78)91054-5.
The distribution of protein-bound labelled leucine in cortical slices prepared from young rats was investigated by autoradiography and complemented with measurements of the rate of protein synthesis in slices. During the second and third postnatal weeks the rate of protein synthesis in cortical slices from rat brain declined rapidly. The distribution of protein-bound radioactivity was fairly homogenous within the neuronal cell population of cortical slices from 7-day-old rats and was not influenced by the level of labelled leucine in the incubation medium (0.01 and 1 mM). On the contrary, the gradient-like distribution of incorporated radioactivity appeared in slices from 14-day-old rats, at both trace and 1 mM levels of the precursor. The laminar analysis of neuronal cell labelling confirmed the preponderance of incorporation into the neurones localized near the slice surface and the considerable inhibition of neuronal protein synthesis in the slice centre. Therefore, these limitations on the use of cortical slices for the study of brain protein synthesis in the rat should be respected from the beginning of the second postnatal week.
通过放射自显影研究了从幼鼠制备的皮质切片中蛋白质结合标记亮氨酸的分布，并辅以切片中蛋白质合成速率的测量。在出生后的第二和第三周，大鼠脑皮质切片中的蛋白质合成速率迅速下降。在7日龄大鼠皮质切片的神经元细胞群体中，蛋白质结合放射性的分布相当均匀，并且不受孵育培养基中标记亮氨酸水平（0.01和1 mM）的影响。相反，在14日龄大鼠的切片中，在前体的痕量和1 mM水平下均出现了掺入放射性的梯度样分布。神经元细胞标记的层分析证实，掺入主要发生在切片表面附近的神经元中，并且切片中心的神经元蛋白质合成受到相当大的抑制。因此，从出生后第二周开始就应注意这些对使用皮质切片研究大鼠脑蛋白质合成的限制。
