Gebicke-Härter P J, Althaus H H, Schwartz P, Neuhoff V
Brain Res. 1981 Jul;227(4):497-518. doi: 10.1016/0165-3806(81)90004-3.
Oligodendrocytes isolated in bulk from white matter of cat brain (8-12 weeks of age) employing a Percoll gradient as the final purification step, were cultured and maintained for more than 10 weeks. Different parameters, e.g. coating material and the age of the animals appeared to have some influence on attachment rate and survival of the cells. Oligodendrocytes from older animals, or oligodendrocytes seeded into poly-L-lysine coated culture dishes revealed a marked tendency to form aggregates. Of the dispersed cells, 80-99% can be classified as oligodendrocytes by transmission electron microscopy (TEM) and immunocytochemical markers. Aggregates which were re-seeded consisted of more than 90% oligodendrocytes. About one day after attachment to the supporting layer the cells start to regenerate their processes which sometimes broaden at their ends into shovel-like, membranous extensions.
从8至12周龄猫脑白质中批量分离出的少突胶质细胞，采用Percoll梯度作为最终纯化步骤，进行培养并维持10周以上。不同参数，如包被材料和动物年龄，似乎对细胞的附着率和存活率有一定影响。来自老年动物的少突胶质细胞，或接种到聚-L-赖氨酸包被培养皿中的少突胶质细胞，显示出明显的聚集倾向。通过透射电子显微镜（TEM）和免疫细胞化学标记，分散细胞中80 - 99%可归类为少突胶质细胞。重新接种的聚集体中少突胶质细胞占比超过90%。附着于支持层约一天后，细胞开始再生其突起，这些突起有时在末端会变宽形成铲状的膜状延伸。
