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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Lake P E, Ravie O, McAdam J

Br Poult Sci. 1981 Jan;22(1):71-7. doi: 10.1080/00071688108447865.

A method of freezing semen of individual males was adapted for use under farm conditions using an automated freezing apparatus. 2. An insemination programme to produce high fertility and hatchability with semen which had been deep frozen for 2 months was devised. 3. Over 90 % fertile eggs with a 90 % hatch of all eggs set was obtained with frozen and thawed semen over a period from the 2 nd to the 12th day after the first of four insemination. The persistency of fertility was also tested and 93, 86.6 and 30.7 % of the eggs were fertile during days 2 to 6, 2 to 8 and 9 to 15 after the last insemination. 4. Corresponding with the high fertility rate, chicks were produced by every hen that was inseminated and from every male whose semen was frozen and stored. The implications for future breeding practices of this successful result are discussed.

一种冷冻单个雄性精液的方法经过调整，可在农场条件下使用自动冷冻设备进行。2. 设计了一个授精方案，使用深度冷冻2个月的精液来实现高受精率和孵化率。3. 在四次授精中的第一次授精后的第2天至第12天期间，使用冷冻和解冻后的精液获得了超过90%的可育卵，所有设置的卵的孵化率为90%。还测试了受精的持续性，在最后一次授精后的第2至6天、第2至8天和第9至15天，分别有93%、86.6%和30.7%的卵受精。4. 与高受精率相对应，每只接受授精的母鸡以及每只精液被冷冻保存的雄性都产出了雏鸡。讨论了这一成功结果对未来育种实践的影响。

Lake P E, Ravie O, McAdam J

Br Poult Sci. 1981 Jan;22(1):71-7. doi: 10.1080/00071688108447865.

A method of freezing semen of individual males was adapted for use under farm conditions using an automated freezing apparatus. 2. An insemination programme to produce high fertility and hatchability with semen which had been deep frozen for 2 months was devised. 3. Over 90 % fertile eggs with a 90 % hatch of all eggs set was obtained with frozen and thawed semen over a period from the 2 nd to the 12th day after the first of four insemination. The persistency of fertility was also tested and 93, 86.6 and 30.7 % of the eggs were fertile during days 2 to 6, 2 to 8 and 9 to 15 after the last insemination. 4. Corresponding with the high fertility rate, chicks were produced by every hen that was inseminated and from every male whose semen was frozen and stored. The implications for future breeding practices of this successful result are discussed.

一种冷冻单个雄性精液的方法经过调整，可在农场条件下使用自动冷冻设备进行。2. 设计了一个授精方案，使用深度冷冻2个月的精液来实现高受精率和孵化率。3. 在四次授精中的第一次授精后的第2天至第12天期间，使用冷冻和解冻后的精液获得了超过90%的可育卵，所有设置的卵的孵化率为90%。还测试了受精的持续性，在最后一次授精后的第2至6天、第2至8天和第9至15天，分别有93%、86.6%和30.7%的卵受精。4. 与高受精率相对应，每只接受授精的母鸡以及每只精液被冷冻保存的雄性都产出了雏鸡。讨论了这一成功结果对未来育种实践的影响。