van den Hurk R, Slof G A
Cell Tissue Res. 1981;218(3):487-97. doi: 10.1007/BF00210109.
Gonadal sex differentiation in rainbow trout takes place between day (D) 45 and D 55 after fertilization. Until D 400 the male GSI is maintained at about 0.4%, and that of the female at about 1%. Treatment with N,N-dimethylformamide (DMF) influences the sex ratio and GSI, and is therefore unsuitable as a solvent for steroids to be added to the aquarium water for examination of their effects on gonadal sex differentiation. Triton X-100 has no such effect, and is used as the steroid solvent in the present experiments. Progesterone (300 microgram/l) administered for 4 weeks from hatching, or from D 43, significantly affects the sex ratio in favour of females. Methyltestosterone given from hatching results in sterilization of the gonads, especially when the treatment is carried out for 8 weeks, or when a relatively high dose (300 microgram/l) is used for 4 weeks. When given from D 43 methyltestosterone has a masculinizing effect. It is concluded that progesterone and androgen(s) are important in inducing the onset of ovarian and testis development, respectively.
虹鳟的性腺性别分化发生在受精后的第45天至第55天之间。直到第400天，雄性的性腺成熟系数（GSI）维持在约0.4%，雌性的则维持在约1%。用N,N-二甲基甲酰胺（DMF）处理会影响性别比例和GSI，因此不适合作为将类固醇添加到水族箱水中以检查其对性腺性别分化影响的溶剂。吐温X-100没有这种影响，在本实验中用作类固醇溶剂。从孵化时起或从第43天起给予4周的孕酮（300微克/升）会显著影响性别比例，有利于雌性。从孵化时起给予甲基睾酮会导致性腺不育，尤其是当处理持续8周时，或者当使用相对高剂量（300微克/升）4周时。从第43天起给予甲基睾酮有雄性化作用。得出的结论是，孕酮和雄激素分别在诱导卵巢和睾丸发育的起始中起重要作用。
