Vojtísková M, Hilgertová J, Dráber P
Folia Biol (Praha). 1981;27(3):203-8.
Androgen receptors were found both in cytosol prepared from intact thymuses of the adult castrated B10. A male and in cytosol from thymuses of the castrated males that had been previously given whole-body irradiation with 6.0 Gy (60Co). Histologically, these thymuses were represented by the reticuloepithelial component, no lymphocytes were found in the cortex and small numbers of lymphocytes remained in the medulla. The 3H-dihydrotestosterone-receptor complex sedimented in the 4S region, as revealed by the 5-20% sucrose gradient centrifugation in buffer containing 0.4 M KC1. Free androgen and non-specifically bound androgen were removed by specific antibody coupled to CNBr-activated Sepharose-4B.
在成年去势B10.A雄性小鼠完整胸腺制备的胞质溶胶中，以及在先前接受6.0 Gy（60Co）全身照射的去势雄性小鼠的胸腺胞质溶胶中均发现了雄激素受体。组织学上，这些胸腺以网状上皮成分为主，皮质中未发现淋巴细胞，髓质中仅残留少量淋巴细胞。在含0.4 M KCl的缓冲液中进行5-20%蔗糖梯度离心显示，3H-二氢睾酮受体复合物在4S区域沉淀。通过偶联到溴化氰活化的琼脂糖-4B上的特异性抗体去除游离雄激素和非特异性结合的雄激素。
