Dumanova L, Karadimov M
Vet Med Nauki. 1978;15(3):100-6.
The changes are followed up in the properties of the macrophage membrane after treatment with phospholipase C and the activity of the glucose-6-phosphate dehydrogenase enzyme in order to elucidate the link between the conformation changes observed and some metabolic prosesses in antigen-stimulated macrophages. The studies were carried out on peritoneal macrophages of guinea pig-treated in vitro with phospholipase C. It was found that: 1. The action of phospholipase C essentially changes the linking parameters available for the fluorochrome 1-aniline-8-naphthalin sulfonic acid. 2. The activity of the glucose-6-phosphate dehydrogenase rises by 34.5% in the phospholipase C-treated macrophages. The fast changes observed in the conformation of the membrane that was studied under the effect of the fluorescence stain AHC, the higher activity of the dehydrogenase enzyme used after phospholipase C treatment, the fact that phospholipase C failed to pass through the macrophage membrane, and the capacity of the latter enzyme to bring about the same metabolic changes in the stimulation of the hexosomonophosphate cycle of the normal macrophages as well as the antigen stimulation produced made it reasonable to believe that the conformation changes produced a 'signal' that is transmitted through the cell membrane, and hence the metabolic changes that followed in the metabolic processes.
用磷脂酶C处理后，对巨噬细胞膜的特性以及葡萄糖-6-磷酸脱氢酶的活性进行跟踪，以阐明在抗原刺激的巨噬细胞中观察到的构象变化与某些代谢过程之间的联系。研究是在体外经磷脂酶C处理的豚鼠腹腔巨噬细胞上进行的。结果发现：1. 磷脂酶C的作用本质上改变了荧光染料1-苯胺-8-萘磺酸可用的连接参数。2. 在经磷脂酶C处理的巨噬细胞中，葡萄糖-6-磷酸脱氢酶的活性提高了34.5%。在荧光染料AHC的作用下，所研究的膜构象中观察到的快速变化、磷脂酶C处理后所用脱氢酶的较高活性、磷脂酶C未能穿过巨噬细胞膜这一事实，以及该酶在刺激正常巨噬细胞的己糖单磷酸循环以及产生抗原刺激时能够引起相同代谢变化，这些都使得有理由相信构象变化产生了一个通过细胞膜传递的“信号”，进而导致了代谢过程中的代谢变化。
