通过暴露于小鼠肝炎病毒（MHV - 4，JHM）在体外诱导小鼠脑内皮细胞和星形胶质细胞产生白细胞介素 - 6 。
Interleukin-6 induction in vitro in mouse brain endothelial cells and astrocytes by exposure to mouse hepatitis virus (MHV-4, JHM).
作者信息
Joseph J, Grun J L, Lublin F D, Knobler R L
机构信息
Department of Neurology, Jefferson Medical College, Philadelphia, PA 19107.
出版信息
J Neuroimmunol. 1993 Jan;42(1):47-52. doi: 10.1016/0165-5728(93)90211-g.
Abstract
Interleukin-6 (IL-6) induction, as detected by bioassay and Northern analysis, was examined in vitro in endothelial cells or astrocytes derived from BALB/c (susceptible) or SJL (resistant) mice following exposure to mouse hepatitis virus (MHV-4) or UV inactivated MHV-4 (UV-MHV-4). In BALB/c endothelial cells, up to 16-fold more IL-6 (> 640 U/ml) was induced, compared to SJL cells which showed a minimal response (40 U/ml), relative to basal levels (< 20 U/ml). In contrast, both BALB/c and SJL astrocytes showed a substantial IL-6 response to MHV-4 and UV-MHV-4 exposure, although a strain difference persisted. Despite strain and cell specific differences in released IL-6, equivalent levels of IL-6 mRNA were induced in all cell types following exposure to MHV-4 or UV-MHV-4.
摘要
通过生物测定法和Northern分析检测白细胞介素-6（IL-6）的诱导情况，在体外对源自BALB/c（易感）或SJL（抗性）小鼠的内皮细胞或星形胶质细胞进行检测，这些细胞在暴露于小鼠肝炎病毒（MHV-4）或紫外线灭活的MHV-4（UV-MHV-4）后进行检测。在BALB/c内皮细胞中，与SJL细胞相比，诱导产生的IL-6（> 640 U/ml）高达16倍，SJL细胞相对于基础水平（< 20 U/ml）显示出最小反应（40 U/ml）。相比之下，BALB/c和SJL星形胶质细胞在暴露于MHV-4和UV-MHV-4后均显示出显著的IL-6反应，尽管品系差异仍然存在。尽管释放的IL-6存在品系和细胞特异性差异，但在暴露于MHV-4或UV-MHV-4后，所有细胞类型中诱导产生的IL-6 mRNA水平相当。
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