Bissonnette J M, Hohimer A R, Richardson B S
J Appl Physiol Respir Environ Exerc Physiol. 1981 Apr;50(4):880-3. doi: 10.1152/jappl.1981.50.4.880.
Methods have been developed for the placement of cannulas in the lateral cerebral ventricle and cisternum magnum of the fetal lamb. Three to seven days after surgery, perfusion of the cerebral ventricular system was carried out in unanesthetized lambs in utero. Artificial cerebrospinal fluid (CSF) was perfused at 123 microliter/min for 4-5 h with no change in fetal arterial O2 pressure, CO2 pressure, pH, mean arterial blood pressure, or heart rate. The preparation is suitable for measurements of net formation of fetal central CSF and also for producing changes in the ionic composition of the fetal CSF.
已经开发出将套管插入胎羊侧脑室和枕大池的方法。术后3至7天，在未麻醉的子宫内胎羊中进行脑室系统灌注。以123微升/分钟的速度灌注人工脑脊液（CSF）4至5小时，胎羊动脉血氧分压、二氧化碳分压、pH值、平均动脉血压或心率均无变化。该制备方法适用于测量胎儿中枢脑脊液的净生成量，也适用于改变胎儿脑脊液的离子组成。
