Shears S B, Bronk J R
J Bioenerg Biomembr. 1980 Dec;12(5-6):379-93. doi: 10.1007/BF00748766.
Liver mitochondria isolated from rats 24 h after a single subcutaneous injection of 8 mg thyroxine per kilogram body weight were compared with those isolated from control rats that received injections of isotonic saline at the same time. The mitochondria isolated from the thyroxine-treated rats show higher rates of energy-dependent K+ and phosphate accumulation than those from control animals. It was also found that mitochondria from the hormone-treated animals required a larger addition of Ca2+/mg mitochondrial protein in order to uncouple oxidative phosphorylation, and showed smaller tendency to swell in vitro under energizing conditions. The data obtained on ion movements support previous observations that the stimulation of the basal rate of mitochondrial respiration by thyroxine is associated with an increase in the transmembrane protonic electrochemical potential difference, and indicate the in vivo the hormone raises the intramitochondrial concentration of K+ and phosphate.
将每千克体重单次皮下注射8毫克甲状腺素24小时后的大鼠肝脏线粒体，与同时注射等渗盐水的对照大鼠的肝脏线粒体进行比较。从甲状腺素处理的大鼠中分离出的线粒体，其能量依赖性钾离子和磷酸盐积累速率高于对照动物的线粒体。还发现，激素处理动物的线粒体需要添加更多的钙离子/毫克线粒体蛋白才能使氧化磷酸化解偶联，并且在体外通电条件下肿胀的趋势较小。关于离子运动获得的数据支持了先前的观察结果，即甲状腺素对线粒体基础呼吸速率的刺激与跨膜质子电化学势差的增加有关，并表明在体内该激素会提高线粒体内钾离子和磷酸盐的浓度。
