Schmidt W N, Pardue R L, Tutt M C, Briggs R C, Brinkley B R, Hnilica L S
Proc Natl Acad Sci U S A. 1982 May;79(10):3138-42. doi: 10.1073/pnas.79.10.3138.
Further characterization of three polypeptide antigens present in Novikoff ascites hepatoma (molecular weights 39,000, 49,000, and 56,000--p39, p49, and p56, respectively), has established the identity of these proteins as cytokeratins. Two-dimensional gel electrophoresis revealed that each of the three proteins exists in several isoelectric forms, all characteristic of the keratins. The antigens p39, and p56 had amino acid compositions compatible with this family of proteins, and immunofluorescence microscopy localized the antigens on elaborate filament arrays indicative of the cytokeratins. Antibodies monospecific for the p39 antigen, purified by using affinity chromatography, were used to demonstrate that protein p39 was specific for Novikoff hepatoma cells. These results suggest that the cytokeratins contain cell-specific that can be useful as markers for differentiation and neoplasia.
对诺维科夫腹水肝癌中存在的三种多肽抗原（分子量分别为39,000、49,000和56,000——分别为p39、p49和p56）的进一步表征，确定了这些蛋白质为细胞角蛋白。二维凝胶电泳显示，这三种蛋白质中的每一种都以几种等电形式存在，所有这些都是角蛋白的特征。抗原p39和p56的氨基酸组成与该蛋白质家族相符，免疫荧光显微镜检查将抗原定位在表明细胞角蛋白的精细丝状阵列上。通过亲和层析纯化的对p39抗原具有单特异性的抗体，用于证明蛋白质p39对诺维科夫肝癌细胞具有特异性。这些结果表明，细胞角蛋白包含细胞特异性成分，可用作分化和肿瘤形成的标志物。
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