Junker S
J Cell Sci. 1981 Feb;47:207-26. doi: 10.1242/jcs.47.1.207.
Pigmented subtetraploid subhexaploid mouse melanoma cells were fused with a range of different cell types. Expression of pigment formation appeared to be dependent on the phenotype of the non-melanoma parent cell, so that hybrids with lymphoid cells or chick embryo erythrocytes produced pigment, but hybrids between fibroblasts or epithelial rat hepatoma cells did not. The results were independent of gene dosage of either parent cell. gamma-irradiation of suppressing partner cells prior to fusion caused progressive increase in pigmentation with increasing dose of radiation. Cybrids between cytoplasts of suppressing fibroblasts and melanoma cells were pigmented.
色素沉着的亚四倍体亚六倍体小鼠黑色素瘤细胞与一系列不同的细胞类型进行了融合。色素形成的表达似乎取决于非黑色素瘤亲本细胞的表型，因此与淋巴细胞或鸡胚红细胞形成的杂种会产生色素，但成纤维细胞或上皮大鼠肝癌细胞之间的杂种则不会。结果与任何一个亲本细胞的基因剂量无关。在融合前对抑制性伙伴细胞进行γ射线照射，随着辐射剂量的增加，色素沉着会逐渐增加。抑制性成纤维细胞的细胞质体与黑色素瘤细胞之间的胞质杂种会产生色素。
