Eichhorst O, Hinderling P H
J Chromatogr. 1981 Jun 12;224(1):67-93. doi: 10.1016/s0378-4347(00)80139-5.
A specific assay method for radiolabelled digoxin and its known apolar metabolites in plasma, urine and saliva was developed. The assay permits the delineation of the pharmacokinetics of digoxin and its metabolites after single-dose administration of the drug to humans. Column chromatographic and solvent extraction procedures were used for the separation of apolar and polar compounds. Thin-layer chromatography was applied for the individual and specific assessment of digoxin and its apolar metabolites. Apolar and polar standards were used for quantitative assessments of all the procedures used. Accuracy and precision of the assay developed were evaluated in plasma, urine and saliva using biological samples spiked with known amounts of standards and by measuring replicates of biological samples obtained from pharmacokinetic studies wit digoxin administration to humans.
开发了一种用于检测血浆、尿液和唾液中放射性标记地高辛及其已知非极性代谢物的特定分析方法。该分析方法能够描绘出地高辛及其代谢物在单剂量给药于人体后的药代动力学情况。采用柱色谱和溶剂萃取程序分离非极性和极性化合物。薄层色谱法用于对地高辛及其非极性代谢物进行单独和特异性评估。使用非极性和极性标准品对所有使用的程序进行定量评估。通过向生物样品中加入已知量的标准品并测量从地高辛给药于人体的药代动力学研究中获得的生物样品复制品，在血浆、尿液和唾液中评估所开发分析方法的准确性和精密度。
