Gault M H, Ahmed M, Symes A L, Vance J
Clin Biochem. 1976 Feb;9(1):46-52. doi: 10.1016/s0009-9120(76)80013-6.
The efficiency with which serveral solvent systems extract digoxin and its metabolites from urine has been studied and column chromatography using Sephadex LH-20 has been used to separate digoxin and its metabolites. These procedures have been evaluated and used to study the excretion of 3H-digoxin-12alpha and its metabolites in urines collected serially in 7 patients and in bile in one. The percentage of the radioactivity excreted as metabolites in urine and bile was found to reach a peak within the first day and then to gradualy decline to minimal amounts in patients with advanced renal failure as well as those with good renal function. The maximum percentage measured as metabolites in 4 patients with the most advanced renal failure was 10%.
研究了几种溶剂系统从尿液中提取地高辛及其代谢物的效率，并采用葡聚糖凝胶LH - 20柱色谱法分离地高辛及其代谢物。对这些方法进行了评估，并用于研究7例患者连续收集的尿液以及1例患者胆汁中3H - 地高辛 - 12α及其代谢物的排泄情况。发现在肾衰竭晚期患者以及肾功能良好的患者中，尿液和胆汁中以代谢物形式排泄的放射性百分比在第一天内达到峰值，然后逐渐下降至最低水平。4例肾衰竭最严重患者中测得的代谢物最大百分比为10%。
