Automated determination of cholesterol by use of immobilized aldehyde dehydrogenase.

A method for the fully enzymic determination of cholesterol by use of aldehyde dehydrogenase, immobilized onto nylon-tubing, is described. The method gives good results, correlates well with enzymic cholesterol determination to solution, and is high in precision. More than 4000 tests can be carried out with one reactor, reducing the cost for aldehyde dehydrogenase to a negligible amount.