Hinsch W, Antonijewić A, Sundaram P V
Clin Chem. 1980 Nov;26(12):1652-5.
We describe routine methods for determining glucose in plasma with use of aldehyde dehydrogenase or glucose oxidase-aldehyde dehydrogenase immobilized in a nylon tube that is integrated into a continuous-flow system. Although the coupled-enzyme nylon-tube reactors require the presence of a third enzyme, catalase, in solution, the kinetics are not so complicated as to preclude reliable routine determination of glucose at very low cost. Precision is good, and results correlate well with those by the method involving glucose oxidase in solution. More than 3000 tests may be carried out with one reactor. The immobilized enzymes are stable for several months at 4 degrees C when not in use.
我们描述了使用固定在尼龙管中的醛脱氢酶或葡萄糖氧化酶 - 醛脱氢酶来测定血浆中葡萄糖的常规方法，该尼龙管集成在连续流动系统中。尽管偶联酶尼龙管反应器需要在溶液中存在第三种酶即过氧化氢酶，但动力学并不复杂，以至于可以以非常低的成本进行可靠的葡萄糖常规测定。精密度良好，结果与溶液中葡萄糖氧化酶方法的结果相关性良好。一个反应器可进行超过3000次测试。固定化酶在不使用时于4℃下可稳定保存数月。
