Sugino W M, Wek R C, Kingsbury D T
J Gen Microbiol. 1980 Dec;121(2):333-8. doi: 10.1099/00221287-121-2-333.
The nucleotide sequence relationships between 18 species of Mycoplasma and 3 species of Acholeplasma were examined by solution DNA hybridization. Radiolabeled DNAs from strains representing 13 Mycoplasma and 2 Acholeplasma species were used as hybridization probes. The mycoplasmas were heterogeneous but displayed a low degree of conserved information of the order of 3 to 5% of the genome. However, several species within each genus showed 13 to 15% homology. There was no detectable homology between species from the two genera, and M. pneumoniae and M. neurolyticum appeared to be unrelated to any of the other Mycoplasma species or to each other. These results suggest that it may be possible to isolate genes common to most, if not all, Mycoplasma and Acholeplasma species.
通过溶液DNA杂交检测了18种支原体和3种无胆甾原体之间的核苷酸序列关系。使用来自代表13种支原体和2种无胆甾原体的菌株的放射性标记DNA作为杂交探针。支原体是异质的，但显示出基因组约3%至5%程度的低保守信息。然而，每个属内的几个物种显示出13%至15%的同源性。两个属的物种之间没有可检测到的同源性，并且肺炎支原体和解脲脲原体似乎与任何其他支原体物种或彼此无关。这些结果表明，有可能分离出大多数（如果不是全部）支原体和无胆甾原体物种共有的基因。
