无胆甾原体属物种间的核酸关系。
Nucleic acid relationships among Acholeplasma species.
作者信息
Aulakh G S, Stephens E B, Rose D L, Tully J G, Barile M F
出版信息
J Bacteriol. 1983 Mar;153(3):1338-41. doi: 10.1128/jb.153.3.1338-1341.1983.
Abstract
3H-labeled Acholeplasma DNA probes were generated in vitro by the nick-translation method and used to determine the nucleotide sequence homology among the type strains of the eight currently recognized species of Acholeplasma. Very little nucleotide sequence homology (less than or equal to 18%) was found among the eight species, with heteroduplexes showing at least 12% or more mismatching as determined by thermal elution midpoints. The small amount of nucleotide sequence homology among the eight species indicates that these species are quite distinct and are not closely related to each other genomically.
摘要
通过缺口平移法在体外制备了3H标记的无胆甾原体DNA探针，并用于确定目前公认的8种无胆甾原体模式菌株之间的核苷酸序列同源性。在这8个物种之间发现了极少的核苷酸序列同源性（小于或等于18%），通过热洗脱中点测定，异源双链体显示至少12%或更高的错配。这8个物种之间少量的核苷酸序列同源性表明这些物种非常独特，在基因组上彼此之间没有密切关系。
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