大鼠和猪脑中的促胰液素免疫反应性。

Secretin immunoreactivity in rat and pig brain.

作者信息

Charlton C G, O'Donohue T L, Miller R L, Jacobowitz D M

出版信息

Peptides. 1981;2 Suppl 1:45-9. doi: 10.1016/0196-9781(81)90054-1.

Abstract

Secretin immunoreactivity in rat and pig brain has been identified and characterized utilizing a highly specific radioimmunoassay and fractionation on a high pressure liquid chromatographic system reverse phase column. One immunoreactive peak from each brain extract was observed. Secretin immunoreactivity from rat brain and duodenum coelute, but eluted slightly ahead of the immunoreactivity from pig brain and duodenum and from synthetic porcine secretin. Immunoreactive secretin is widely distributed in the thalamus, hypothalamus and olfactory bulb, cerebral cortex, midbrain, septum, striatum, hippocampus, medulla and pons. The highest concentrations occur in the pineal and the pituitary gland.

摘要

利用高度特异性放射免疫分析法以及在高压液相色谱系统反相柱上进行分级分离，已对大鼠和猪脑中的促胰液素免疫反应性进行了鉴定和表征。在每种脑提取物中均观察到一个免疫反应峰。大鼠脑和十二指肠中的促胰液素免疫反应性共同洗脱，但比猪脑、十二指肠以及合成猪促胰液素中的免疫反应性略早洗脱。免疫反应性促胰液素广泛分布于丘脑、下丘脑、嗅球、大脑皮层、中脑、隔区、纹状体、海马体、延髓和脑桥。最高浓度出现在松果体和垂体中。