大鼠和猪脑中促胰液素免疫反应性的鉴定、特征及分布
Identification, characterization, and distribution of secretin immunoreactivity in rat and pig brain.
作者信息
O'Donohue T L, Charlton C G, Miller R L, Boden G, Jacobowitz D M
出版信息
Proc Natl Acad Sci U S A. 1981 Aug;78(8):5221-4. doi: 10.1073/pnas.78.8.5221.
Abstract
Secretin immunoreactivity was detected in the central nervous system of the rat and pig with a highly specific radioimmunoassay. The secretin immunoreactivity in the rat and pig brain and duodenum extracts was fractionated by using a reverse-phase high-pressure liquid chromatographic system. The immunoreactive secretin from pig brain and duodenum coeluted precisely with synthetic porcine secretin. However, immunoreactive secretin extracted from rat brain and duodenum eluted slightly before porcine secretin. These data suggest a slight difference in the structure of rat and pig secretin. The detection of secretin in the brain lays the groundwork for studies to determine the role of the peptide in central nervous system function.
摘要
采用高特异性放射免疫分析法在大鼠和猪的中枢神经系统中检测到了促胰液素免疫反应性。利用反相高压液相色谱系统对大鼠和猪脑及十二指肠提取物中的促胰液素免疫反应性进行了分离。猪脑和十二指肠中的免疫反应性促胰液素与合成猪促胰液素精确地共洗脱。然而，从大鼠脑和十二指肠中提取的免疫反应性促胰液素在猪促胰液素之前稍有洗脱。这些数据表明大鼠和猪促胰液素的结构存在细微差异。在脑中检测到促胰液素为确定该肽在中枢神经系统功能中的作用的研究奠定了基础。
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