Smith M T, Thor H, Orrenius S
Science. 1981 Sep 11;213(4513):1257-9. doi: 10.1126/science.7268433.
Freshly isolated hepatocytes from phenobarbital-treated rats were incubated in the presence or absence of extracellular calcium with three differently acting liver cell toxins, namely carbon tetrachloride, bromobenzene, and ethylmethanesulfonate. In the absence of extracellular calcium these three compounds were far more toxic to the cells than in its presence. This result is inconsistent with the hypothesis that an influx of extracellular calcium is required as the final step in toxic liver cell injury.
将从经苯巴比妥处理的大鼠中新鲜分离出的肝细胞，在有或无细胞外钙存在的情况下，与三种作用方式不同的肝细胞毒素一起孵育，这三种毒素分别是四氯化碳、溴苯和甲基磺酸乙酯。在无细胞外钙的情况下，这三种化合物对细胞的毒性远大于有细胞外钙存在时。这一结果与下述假设不一致，该假设认为细胞外钙内流是毒性肝细胞损伤的最后一步所必需的。
