Fariss M W, Pascoe G A, Reed D J
Science. 1985 Feb 15;227(4688):751-4. doi: 10.1126/science.3918345.
Isolated rat hepatocytes were incubated in the presence or absence of extracellular calcium and alpha-tocopherol succinate with three different toxic chemicals; namely, adriamycin in combination with 1,3-bis(2-chloroethyl)-1-nitrosourea, ethyl methanesulfonate, and the calcium ionophore A23187. In the absence of extracellular calcium these three compounds were far more toxic to the cells than in its presence. The addition of vitamin E to calcium-free medium, however, protected hepatocytes against toxic injury, whereas cells incubated in medium containing calcium were not protected. Hepatocyte viability during each toxic insult correlated well with the cellular alpha-tocopherol content but not with the presence or absence of extracellular calcium. These results suggest that cellular alpha-tocopherol maintains the viability of the cell during a toxic insult and that the presence or absence of vitamin E in the incubation medium probably explains the conflicting reports on the role of extracellular calcium in toxic cell death.
将分离的大鼠肝细胞与三种不同的有毒化学物质一起在有或没有细胞外钙和琥珀酸生育酚的情况下孵育；即阿霉素与1,3 - 双(2 - 氯乙基)-1 - 亚硝基脲、甲磺酸乙酯和钙离子载体A23187联合使用。在没有细胞外钙的情况下，这三种化合物对细胞的毒性比有细胞外钙时大得多。然而，在无钙培养基中添加维生素E可保护肝细胞免受毒性损伤，而在含钙培养基中孵育的细胞则未得到保护。每次毒性损伤期间肝细胞的活力与细胞内生育酚含量密切相关，而与细胞外钙的有无无关。这些结果表明，细胞内生育酚在毒性损伤期间维持细胞活力，并且孵育培养基中维生素E的有无可能解释了关于细胞外钙在毒性细胞死亡中作用的相互矛盾的报道。
