Sakuma H, Asano S, Kojima M
Acta Pathol Jpn. 1981 May;31(3):473-93. doi: 10.1111/j.1440-1827.1981.tb01389.x.
The present authors have studied the cellular composition of the primary follicle (PF) in the germ-free rat lymph nodes and the development of PF in the human lymph node anlages by using the light- and the electron microscopy. According to the ultrastructural study, the cellular composition of PF in the germ-free rat lymph nodes and classified into five cell types, 1) lymphocytic cells (small and medium-sized lymphocytes), 2) large lymphocytes (= large germinal center cells), 3) reticulum cells with desmosomes (RED), 4) desmodendric cells (DDC), and 5) histiocytes. The interstitial cells with desmosome are classified into DDC and RED based on the relationship to collagenous fibers and the morphological differences. There are amorphous electron-dense intercellular materials among the cytoplasmic processes of DDC, which are distinguished from histiocytes based on the morphological differences. PF emerges as a spherical aggregation of young DDC straight underneath the marginal sinus in the outer cortex of the human embryonal lymph node anlages on the 16th fetal week.
作者通过光学显微镜和电子显微镜研究了无菌大鼠淋巴结中初级滤泡（PF）的细胞组成以及人淋巴结原基中PF的发育情况。根据超微结构研究，无菌大鼠淋巴结中PF的细胞组成可分为五种细胞类型：1）淋巴细胞（小淋巴细胞和中淋巴细胞）；2）大淋巴细胞（=大生发中心细胞）；3）有桥粒的网状细胞（RED）；4）桥粒树突状细胞（DDC）；5）组织细胞。有桥粒的间质细胞根据与胶原纤维的关系和形态差异分为DDC和RED。DDC的细胞质突起之间存在无定形电子致密细胞间物质，根据形态差异可将其与组织细胞区分开来。在胎儿第16周时，PF在人胚胎淋巴结原基外皮质的边缘窦正下方以年轻DDC的球形聚集形式出现。
