Müller-Lissner S A, Schattenmann G, Schenker G, Sonnenberg A, Hollinger A, Siewert J R, Blum A L
Am J Physiol. 1981 Aug;241(2):G159-62. doi: 10.1152/ajpgi.1981.241.2.G159.
A two-marker technique was used to determine duodenogastric reflux in fasting dogs with normal or surgically modified gastroduodenal junctions. All nine dogs had an esophagostomy for gastric marker perfusion. The duodenal marker was given via a duodenal fistula. In two dogs a Heineke-Mikulicz pyloroplasty was performed, and in four dogs extramucosal circular pylorectomy was performed in addition. The mean fasting duodenogastric reflux rate in dogs with a normal pylorus was 1.1 +/- 0.5 (SE) ml/10 min; after pyloroplasty it was 1.6 +/- 0.3 ml/10 min (P greater than 0.1), and after pylorectomy it was 1.5 +/- 0.4 ml/10 min (P greater than 0.1). Simultaneous intraduodenal manometry revealed no relation between the interdigestive myoelectric complex and reflux. The marker technique for the measurement of reflux was validated by pharmacologically induced reflux. Subcutaneous injection of 0.1 mg of apomorphine increased the reflux rate tenfold. A transpyloric tube increased reflux rate fivefold. It is concluded that, in the fasting dog, phenomena such as retropulsive peristalsis are determinants of duodenogastric reflux and not the presence or absence of the pylorus and normal interdigestive motility.
采用双标记技术测定空腹状态下胃十二指肠连接部正常或经手术改变的犬的十二指肠胃反流情况。所有9只犬均行食管造口术用于胃标记物灌注。十二指肠标记物通过十二指肠瘘给予。对2只犬进行了海涅克-米库利奇幽门成形术，另外4只犬还进行了黏膜外环形幽门切除术。幽门正常的犬的平均空腹十二指肠胃反流率为1.1±0.5（标准误）ml/10分钟；幽门成形术后为1.6±0.3 ml/10分钟（P>0.1），幽门切除术后为1.5±0.4 ml/10分钟（P>0.1）。同时进行的十二指肠内压力测定显示消化间期肌电复合波与反流之间无关联。通过药理学诱导反流验证了测量反流的标记技术。皮下注射0.1 mg阿扑吗啡可使反流率增加10倍。经幽门管可使反流率增加5倍。得出的结论是，在空腹犬中，诸如逆蠕动等现象是十二指肠胃反流的决定因素，而非幽门的存在与否以及正常的消化间期运动。
