Chien P T
Am Ind Hyg Assoc J. 1978 Oct;39(10):808-16. doi: 10.1080/0002889778507860.
An ultra-sensitive fluorometric method has been developed for quantitating subtilisins in aqueous solution. Using detergent solutions as blanks, the analytical sensitivity was estimated to be 0.002 microgram of 100% active enzyme per milliliter. The required sampling time for quantitating subtilisins in air at their TLV was estimated to be approximately two minutes.
