Webster J H, Baird J D, Gunawan M, Martin I C, Kelly J D
Aust Vet J. 1981 Apr;57(4):172-81. doi: 10.1111/j.1751-0813.1981.tb00504.x.
The susceptibility of a known thiabendazole-resistant population of small strongyles to anthelmintics of both benzimidazole and non-benzimidazole groups, was determined. In the first study, 42 horses infected with thiabendazole-resistant small strongyles were allocated to 6 groups. Treatment groups received one of the following anthelmintics: mebendazole, febantel, febantel plus trichlorphon, morantel tartrate, or a combination of thiabendazole, piperazine and trichlorphon. Morantel tartrate and the thiabendazole/piperazine/trichlorphon combination produced highly significant (p less than 0.001) reductions in faecal strongyle egg counts 20 days post-treatment. Mebendazole, febantel and febantel plus trichlorphon failed to reduce strongyle egg counts significantly. Larval culture and differentiation indicated that in all cases of anthelmintic failure, small strongyles of the sub-family Cyathostominae were involved. Eighteen horses from groups in which treatment had failed were re-allocated to 3 groups. Treatment with either morantel tartrate or haloxon was highly efficient in reducing faecal strongyle egg counts. In the final study, fifty-four horses, infected with benzimidazole-resistant small strongyles were allocated to 10 groups. On day zero, each treatment group received one of the following anthelmintics: thiabendazole, cambendazole, mebendazole, oxibendazole, piperazine, thiabendazole/piperazine, cambendazole/piperazine, mebendazole/piperazine or oxibendazole/piperazine. Oxibendazole, piperazine and the benzimidazole/piperazine combinations produced highly significant reductions in faecal strongyle egg counts 20 days post-treatment (p less than 0.001). When administered alone, benzimidazole anthelmintics failed to reduce strongyle egg counts significantly, with the exception of oxibendazole. Larval culture and differentiation indicated that in all cases of anthelmintic failure, the species involved were small strongyles of subfamily Cyathostominae. There was no significant increase in benzimidazole resistance level (based on in vitro assay) as a result of drug treatment, over one generation.
测定了已知对噻苯达唑耐药的小型圆线虫群体对苯并咪唑类和非苯并咪唑类驱虫药的敏感性。在第一项研究中，将42匹感染了对噻苯达唑耐药的小型圆线虫的马分为6组。治疗组接受以下驱虫药之一：甲苯咪唑、非班太尔、非班太尔加敌百虫、酒石酸噻嘧啶，或噻苯达唑、哌嗪和敌百虫的组合。酒石酸噻嘧啶和噻苯达唑/哌嗪/敌百虫组合在治疗后20天使粪便中圆线虫卵计数显著降低（p<0.001）。甲苯咪唑、非班太尔和非班太尔加敌百虫未能显著降低圆线虫卵计数。幼虫培养和鉴别表明，在所有驱虫失败的病例中，均涉及杯冠属小型圆线虫。将治疗失败组的18匹马重新分为3组。用酒石酸噻嘧啶或哈罗松治疗在降低粪便中圆线虫卵计数方面非常有效。在最后一项研究中，将54匹感染了对苯并咪唑耐药的小型圆线虫的马分为10组。在第0天，每个治疗组接受以下驱虫药之一：噻苯达唑、坎苯达唑、甲苯咪唑、奥芬达唑、哌嗪、噻苯达唑/哌嗪、坎苯达唑/哌嗪、甲苯咪唑/哌嗪或奥芬达唑/哌嗪。奥芬达唑、哌嗪和苯并咪唑/哌嗪组合在治疗后20天使粪便中圆线虫卵计数显著降低（p<0.001）。单独使用时，除奥芬达唑外，苯并咪唑类驱虫药未能显著降低圆线虫卵计数。幼虫培养和鉴别表明，在所有驱虫失败的病例中，涉及的种类均为杯冠属小型圆线虫。经过一代药物治疗后，苯并咪唑耐药水平（基于体外试验）没有显著增加。
