Sakata T, von Engelhardt W
Cell Tissue Res. 1981;219(3):629-35. doi: 10.1007/BF00210000.
The luminal and epithelial mucin was studied histochemically in the large intestine of mice (Mus musculus), rats (Rattus rattus) and guinea pigs (Cavia porcellus) using freeze-substitution and vapor-fixation methods. Neutral mucin decreased and acid mucin increased in the epithelium from the cecum to the distal colon. Vacuolated cells contained more acid mucin than goblet cells. Luminal mucin always contained neutral mucin, which formed the main constituents in the cecum and in the proximal colon. Sialo-mucin increased from the cecum to the distal colon. Sulfo-mucin appeared only in the distal colon. Except in the cecum a luminal mucin layer (LML) was found at the epithelial surface. In the proximal colon LML was not entirely continuous and varied in composition and thickness (182.4 +/- 170.1, 150.5 +/- 110.4, 30.0 +/- 28.9 (micrometer), in mice, rats and guinea pigs, respectively), and contained many bacteria. In the distal colon LML was compact, homogeneous and thin (33.6 +/- 18.8, 16.1 +/- 7.3, 29.1 +/- 20.0 (micrometer), in mice, rats and guinea pigs, respectively) containing few bacteria. Possible functions of the luminal mucin and their regional differentiations were discussed.
采用冷冻置换法和蒸汽固定法，对小鼠（小家鼠）、大鼠（褐家鼠）和豚鼠（豚鼠）的大肠进行组织化学研究，观察其管腔和上皮黏蛋白情况。从盲肠到结肠远端，上皮中的中性黏蛋白减少，酸性黏蛋白增加。空泡化细胞比杯状细胞含有更多的酸性黏蛋白。管腔黏蛋白始终含有中性黏蛋白，中性黏蛋白是盲肠和近端结肠的主要成分。从盲肠到结肠远端，唾液酸黏蛋白增加。硫酸黏蛋白仅出现在结肠远端。除盲肠外，上皮表面均发现有管腔黏蛋白层（LML）。在近端结肠，LML并不完全连续，其组成和厚度各异（小鼠、大鼠和豚鼠分别为182.4±170.1、150.5±110.4、30.0±28.9（微米）），且含有许多细菌。在远端结肠，LML致密、均匀且薄（小鼠、大鼠和豚鼠分别为33.6±18.8、16.1±7.3、29.1±20.0（微米）），含细菌较少。文中讨论了管腔黏蛋白的可能功能及其区域差异。
