Matsuo K, Ota H, Akamatsu T, Sugiyama A, Katsuyama T
Department of Second Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Gut. 1997 Jun;40(6):782-9. doi: 10.1136/gut.40.6.782.
Histochemical analysis of the surface mucous gel layer of the human colon is difficult, as it dissolves in fixatives. This study was undertaken to explore the surface mucous gel layer on the normal mucosa and neoplastic tissues of the large intestine. In addition, the distribution of different mucins secreted from goblet cells was studied with a series of histochemical stains for mucins.
Twenty four surgically resected specimens were fixed in Carnoy's solution and embedded in paraffin. In four cases, the surface mucous gel layer was also studied in frozen sections. Serial sections were stained by a battery of histochemical techniques characterising mucins.
The surface mucous gel layer consisted of the inner and outer layers. The first covered the luminal surface of the mucosa, consisted of mucins, and showed a vertical striped pattern. The second overlaid the first, showed a lateral striped pattern, and was contaminated with bacteria and other substances. Their thickness in paraffin sections varied considerably among the sites in the large intestine, but was the thickest in the rectum and measured 12.7 (SEM 6.0) microns and 88.8 (SEM 80.1) microns respectively. Mucins forming the inner layer were obviously derived from goblet cells underlying it.
由于人结肠表面黏液凝胶层会溶解于固定剂中，因此对其进行组织化学分析存在困难。本研究旨在探索大肠正常黏膜和肿瘤组织上的表面黏液凝胶层。此外，利用一系列针对黏蛋白的组织化学染色方法，研究了杯状细胞分泌的不同黏蛋白的分布情况。
将24个手术切除标本固定于卡诺氏液中，然后石蜡包埋。其中4例标本还对其冷冻切片进行了表面黏液凝胶层的研究。采用一系列能够鉴定黏蛋白的组织化学技术对连续切片进行染色。
表面黏液凝胶层由内层和外层组成。内层覆盖于黏膜腔面，由黏蛋白构成，呈垂直条纹状。外层覆盖在内层之上，呈横向条纹状，且被细菌和其他物质污染。其在石蜡切片中的厚度在大肠各部位差异很大，但在直肠最厚，分别为12.7（标准误6.0）微米和88.8（标准误80.1）微米。构成内层的黏蛋白明显来源于其下方的杯状细胞。
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