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海胆胚胎中染色体小泡的超微结构及3H-胸腺嘧啶核苷掺入情况

Ultrastructure and 3H-thymidine incorporation by chromosome vesicles in sea urchin embryos.

作者信息

Ito S, Dan K, Goodenough D

出版信息

Chromosoma. 1981;83(4):441-53. doi: 10.1007/BF00328271.

DOI:10.1007/BF00328271
PMID:7273955
Abstract

The ultrastructural features of chromosome vesicle formation in early sea urchin embryos and chromosome vesicle uptake of tritiated thymidine is described. Envelopes which resemble typical nuclear envelopes form around the condensed anaphase chromosomes. In late anaphase or early telophase, the chromosomes swell and decondense and it is at this time when tritiated thymidine is incorporated. This study shows that DNA synthesis in the rapidly dividing cells or early sea urchin embryos occurs in chromosome vesicles which form during anaphase.

摘要

描述了海胆早期胚胎中染色体囊泡形成的超微结构特征以及氚标记胸腺嘧啶核苷的染色体囊泡摄取情况。在浓缩的后期染色体周围形成了类似于典型核膜的包膜。在后期末期或前期早期,染色体膨胀并解聚,正是在这个时候氚标记胸腺嘧啶核苷被掺入。这项研究表明,快速分裂的细胞或海胆早期胚胎中的DNA合成发生在后期形成的染色体囊泡中。

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Ultrastructure and 3H-thymidine incorporation by chromosome vesicles in sea urchin embryos.海胆胚胎中染色体小泡的超微结构及3H-胸腺嘧啶核苷掺入情况
Chromosoma. 1981;83(4):441-53. doi: 10.1007/BF00328271.
2
[Incorporation at [3H]thymidine into the repetitive and unique nucleotide sequences in DNA of the sea urchin Strongylocentrotus droebachiensis at the stage of synchronous cleavage divisions].[在同步卵裂分裂阶段,[3H]胸苷掺入海胆强壮海胆DNA中的重复和独特核苷酸序列]
Biokhimiia. 1982 Jul;47(7):1147-53.
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2
Real time observation of anaphase in vitro.体外后期的实时观察。
Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12327-32. doi: 10.1073/pnas.93.22.12327.
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A monoclonal antibody against the nucleus reveals the presence of a common protein in the nuclear envelope, the perichromosomal region, and cytoplasmic vesicles.

本文引用的文献

1
Electron microscope study of mitosis in sea urchin blastomeres.海胆卵裂球有丝分裂的电子显微镜研究。
J Biophys Biochem Cytol. 1961 Nov;11(2):419-31. doi: 10.1083/jcb.11.2.419.
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Nuclear envelope structure during cell division in Chaetopterus eggs.沙蠋卵细胞分裂过程中的核膜结构。
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3
Ribosomal RNA synthesis and the multiple, atypical nucleoli in cleaving embryos.核糖体RNA合成与分裂胚胎中的多个非典型核仁。
一种针对细胞核的单克隆抗体显示,在核膜、染色体周围区域和细胞质小泡中存在一种共同蛋白。
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Structural analysis of the mitotic cycle in pre-gastrula Xenopus embryos.非洲爪蟾原肠胚形成前胚胎有丝分裂周期的结构分析。
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Ribosomal RNA synthesis in pre- and post-gastrula-stage sea urchin embryos.
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