一种通过气相色谱法和3H-蔗糖测量细胞内水含量的新的、快速且精确的方法。
A new, rapid and precise method for measuring the intra-cellular water content by gas chromatography and 3H-sucrose.
作者信息
Kageyama K, Yamashita T, Kobayashi T, Kimura E
出版信息
Experientia. 1981 Jul 15;37(7):748-50. doi: 10.1007/BF01967959.
PMID:7274387
Abstract
A new rapid method for measuring intra-cellular water content by gas chromatography and an isotopic (3H-sucrose) technique is described. Water content of circulating red cells was revealed to be 71.26 +/- 0.31%. Intra-cellular water content increased to 102.9% of its original value following addition of 3 volumes of acid-citrate-dextrose (ACD) to 20 volumes of blood.
摘要
本文描述了一种通过气相色谱法和同位素（³H-蔗糖）技术测量细胞内含水量的新的快速方法。循环红细胞的含水量为71.26±0.31%。向20份血液中加入3份酸性枸橼酸盐葡萄糖溶液（ACD）后，细胞内含水量增加至其初始值的102.9%。
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