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一种通过气相色谱法和3H-蔗糖测量细胞内水含量的新的、快速且精确的方法。

A new, rapid and precise method for measuring the intra-cellular water content by gas chromatography and 3H-sucrose.

作者信息

Kageyama K, Yamashita T, Kobayashi T, Kimura E

出版信息

Experientia. 1981 Jul 15;37(7):748-50. doi: 10.1007/BF01967959.

DOI:10.1007/BF01967959
PMID:7274387
Abstract

A new rapid method for measuring intra-cellular water content by gas chromatography and an isotopic (3H-sucrose) technique is described. Water content of circulating red cells was revealed to be 71.26 +/- 0.31%. Intra-cellular water content increased to 102.9% of its original value following addition of 3 volumes of acid-citrate-dextrose (ACD) to 20 volumes of blood.

摘要

本文描述了一种通过气相色谱法和同位素（³H-蔗糖）技术测量细胞内含水量的新的快速方法。循环红细胞的含水量为71.26±0.31%。向20份血液中加入3份酸性枸橼酸盐葡萄糖溶液（ACD）后，细胞内含水量增加至其初始值的102.9%。

相似文献

1
A new, rapid and precise method for measuring the intra-cellular water content by gas chromatography and 3H-sucrose.一种通过气相色谱法和3H-蔗糖测量细胞内水含量的新的、快速且精确的方法。
Experientia. 1981 Jul 15;37(7):748-50. doi: 10.1007/BF01967959.
2
Effect of incubation on red cell shape and water content as measured by gas-liquid chromatography.孵育对红细胞形状和水含量的影响（通过气液色谱法测定）。
Nouv Rev Fr Hematol (1978). 1979;21(2):161-9.
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Measurement of adenosine triphosphate and some other metabolites in blood cells by isotachophoresis. II. Application to monitoring changes during storage in acid-citrate-dextrose preservative.用等速电泳法测定血细胞中的三磷酸腺苷及其他一些代谢物。II. 应用于监测在酸性枸橼酸盐葡萄糖保存液中储存期间的变化。
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Effects of treatment with glutaraldehyde and of storage in acid-citrate-dextrose solution on agglutinability of erythrocytes.戊二醛处理及在酸性枸橼酸盐葡萄糖溶液中储存对红细胞凝集性的影响。
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Liquid preservation of baboon red blood cells in acid-citrate-dextrose or citrate-phosphate-dextrose anticoagulant: effects of washing liquid-stored red blood cells.在酸性枸橼酸盐葡萄糖或枸橼酸盐磷酸盐葡萄糖抗凝剂中对狒狒红细胞进行液体保存：洗涤液储存红细胞的影响。
Am J Vet Res. 1981 Jun;42(6):1011-3.
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A follow-up on functional parameters of blood stored in ACD at +4 degrees C.对储存在4℃酸性枸橼酸盐葡萄糖溶液（ACD）中的血液功能参数的随访。
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An improved method for measuring intracellular electrolytes in erythrocytes and the effects of cold storage.一种用于测量红细胞内电解质的改进方法及冷藏的影响。
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A new rapid method for measuring the water content of whole blood, plasma and packed red blood cells by gas chromatography.一种通过气相色谱法测量全血、血浆和红细胞压积水含量的新快速方法。
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Erythrocyte insulin receptors remain intact through three days of storage.红细胞胰岛素受体在储存三天后仍保持完整。
J Endocrinol Invest. 1983 Aug;6(4):301-4. doi: 10.1007/BF03347594.

引用本文的文献

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Moderately controlled transport of ascorbate into aortic endothelial cells against slowdown of the cell cycle, decreasing of the concentration or increasing of coexistent glucose as compared with dehydroascorbate.与脱氢抗坏血酸相比，抗坏血酸适度受控地转运至主动脉内皮细胞，以对抗细胞周期的减慢、浓度的降低或共存葡萄糖的增加。
Mol Cell Biochem. 1997 Aug;173(1-2):43-50. doi: 10.1023/a:1006879001316.

本文引用的文献

1
The distribution of sodium and potassium in oxygenated human blood and their effects upon the movements of water between cells and plasma.钠和钾在含氧人体血液中的分布及其对细胞与血浆间水移动的影响。
Am J Physiol. 1947 May;149(2):340-9. doi: 10.1152/ajplegacy.1947.149.2.340.
2
OSMOTIC PROPERTIES OF HUMAN RED CELLS.人类红细胞的渗透特性。
J Gen Physiol. 1964 Sep;48(1):79-94. doi: 10.1085/jgp.48.1.79.
3
The transport of sodium into human erythrocytes in vivo.体内钠向人体红细胞的转运。
J Gen Physiol. 1955 Jan 20;38(3):389-404. doi: 10.1085/jgp.38.3.389.
4
Intercellular plasma of centrifuged human erythrocytes as measured by means of iodo 131 albumin.
J Appl Physiol. 1953 Jan;5(7):323-9. doi: 10.1152/jappl.1953.5.7.323.
5
Erythrocyte osmotic fragility and cell water--influence of pH and temperature.红细胞渗透脆性与细胞内水分——pH值和温度的影响
J Lab Clin Med. 1969 Aug;74(2):319-24.
6
A new rapid method for measuring the water content of whole blood, plasma and packed red blood cells by gas chromatography.一种通过气相色谱法测量全血、血浆和红细胞压积水含量的新快速方法。
Osaka City Med J. 1977;23(1):61-6.
7
Effect of incubation on red cell shape and water content as measured by gas-liquid chromatography.孵育对红细胞形状和水含量的影响（通过气液色谱法测定）。
Nouv Rev Fr Hematol (1978). 1979;21(2):161-9.