Kageyama K
Nouv Rev Fr Hematol (1978). 1979;21(2):161-9.
A new method for measuring water content of red cells by using gas chromatography is described. Water content of healthy adult red cells was estimated to be 71.73 +/- 0.72%. Changes in water content and red cell shape following incubation are described. Intracellular water content increased in relation to the incubation time, reaching a maximum of 109% at 24 h, and then decreased to 82% at 48 h. Intercellular water content (trapped water) also increased for 24 h, reaching 209%, and then showed no remarkable change until 48 h. Observations with the scanning electron microscope revealed that these changes were associated with the process of transformation to echinocytes, both in numbers and stages.
本文描述了一种利用气相色谱法测量红细胞含水量的新方法。健康成年红细胞的含水量估计为71.73±0.72%。文中描述了孵育后红细胞含水量和形状的变化。细胞内含水量随孵育时间增加，在24小时时达到最大值109%，然后在48小时时降至82%。细胞间含水量（滞留水）在24小时内也增加，达到209%，然后直到48小时都没有显著变化。扫描电子显微镜观察显示，这些变化在数量和阶段上都与向棘状红细胞转变的过程有关。
