Sergeev P V, Belykh A G
Farmakol Toksikol. 1981 Mar-Apr;44(2):195-200.
It has been shown in experiments in vitro that the hepatotrophic organic anions, the radiographic contrast agent (RCA) bilignost used in cholecystography and Bengal pink, have an affinity, unlike the urographic RCA triombrin and renotrophic dye indigo-carmine, for the plasmatic membranes (PM) of liver cells. Hydrophobic interaction has been ascertained to be of primary importance in the course of Bengal pink binding to PM of hepatocytes. PM has a higher capacity for binding Bengal pink as compared to that of lysosomal and microsomal membranes of liver cells. The binding capacity of uterine and renal PM is about 2.5 and 3 times less, respectively than that of liver PM, that is accounted for by differences in the number of binding sites on the membranes with a similar affinity for the dye. The results obtained indicate that PM of liver cells carry specific binding sites responsible for recognition and selective absorption of the hepatotrophic organic anions.
体外实验表明，与用于尿路造影的造影剂三溴酚和肾营养染料靛胭脂不同，用于胆囊造影的促肝细胞有机阴离子、造影剂必利胆显和孟加拉红对肝细胞的质膜具有亲和力。已确定疏水相互作用在孟加拉红与肝细胞质膜结合过程中起主要作用。与肝细胞的溶酶体膜和微粒体膜相比，质膜结合孟加拉红的能力更高。子宫和肾质膜的结合能力分别比肝质膜低约2.5倍和3倍，这是由对该染料具有相似亲和力的膜上结合位点数量的差异所致。所得结果表明，肝细胞质膜带有负责识别和选择性吸收促肝细胞有机阴离子的特异性结合位点。
