Rozinova E N, Mechetner E B, Ievleva E S
Folia Biol (Praha). 1981;27(4):255-64.
The effect of rabbit anti-mouse brain serum and monospecific antibodies to an antigen of erythroblasts, Ag-Eb, on cells forming haemopoietic colonies on glass coverslips was studied. These colonies developed on the fibroblast-macrophage layer formed on glass coverslips which had been inserted into the peritoneal cavity of mice. when bone marrow cell suspensions were treated with RAMBS and the complement, or with anti-Ag-Eb antibodies plus complement, the CFU-GC colony-forming ability remained unaffected. In the examined cell suspensions, RAMBS inactivated 44% of CFU-S. When bone marrow cell suspensions were treated with anti-Ag-Eb antibodies, the number of immature erythroid cells was reduced to 75%. The CFU-GC are presumed to resemble the committed (unipotent) haemopoietic progenitor cells.
研究了兔抗小鼠脑血清和针对成红细胞抗原Ag-Eb的单特异性抗体对在玻璃盖玻片上形成造血集落的细胞的影响。这些集落在插入小鼠腹腔的玻璃盖玻片上形成的成纤维细胞-巨噬细胞层上发育。当用兔抗小鼠脑血清和补体，或用抗Ag-Eb抗体加补体处理骨髓细胞悬液时，CFU-GC集落形成能力不受影响。在所检测的细胞悬液中，兔抗小鼠脑血清使44%的CFU-S失活。当用抗Ag-Eb抗体处理骨髓细胞悬液时，未成熟红细胞的数量减少到75%。推测CFU-GC类似于定向（单能）造血祖细胞。
