Pirofsky B, Beaulieu R, Davies G H
Immunology. 1981 Aug;43(4):653-61.
Extracts produced from the seeds of Ulex europeus (European gorse) were found to contain an inhibitor of human and murine lymphocyte growth. Interference with lymphocyte growth was demonstrated in three test systems. (1) Ulex seed extracts (USE) prevented the uptake of tritiated thymidine by stimulated human T and B lymphocytes. (2) The numbers of sensitized murine lymphocytes releasing antibody were diminished after incubation with USE. (3) Murine lymphoid tumour cells cultured in the presence of USE had a reduced growth potential. Growth inhibition occurred independently of the mode of lymphocyte stimulation. Increased metabolic activity induced by plant seed mitogens, alloantigens and direct immunization, as well as that endogenously present in rapidly growing tumour cells, was similarly restricted. The inhibition of lymphocyte growth was not the result of cytotoxicity and was easily reversible by washing lymphocytes free of USE. The USE growth inhibitor was found not to be anti-H and appeared to function as an antithesis of stimulators found in other plant seed extracts.
研究发现，欧洲荆豆种子提取物含有一种抑制人类和小鼠淋巴细胞生长的物质。在三个测试系统中均证实了其对淋巴细胞生长的干扰作用。（1）欧洲荆豆种子提取物（USE）可阻止受刺激的人类T淋巴细胞和B淋巴细胞摄取氚标记的胸腺嘧啶核苷。（2）与USE孵育后，释放抗体的致敏小鼠淋巴细胞数量减少。（3）在USE存在的情况下培养的小鼠淋巴瘤细胞生长潜力降低。生长抑制的发生与淋巴细胞刺激方式无关。植物种子促细胞分裂剂、同种异体抗原和直接免疫诱导的代谢活性增加，以及快速生长的肿瘤细胞内源性存在的代谢活性增加，同样受到限制。淋巴细胞生长的抑制并非细胞毒性的结果，通过洗涤去除USE后，淋巴细胞的生长抑制作用很容易逆转。研究发现，USE生长抑制剂并非抗H物质，其作用似乎与其他植物种子提取物中的刺激物相反。
