Phair J P, Bassaris H P, Morlock B A
Infect Immun. 1981 Aug;33(2):503-6. doi: 10.1128/iai.33.2.503-506.1981.
Retention of polymorphonuclear leukocytes (PMN) by nylon columns is significantly increased when PMN are suspended in zymosan-activated plasma. In zymosan-activated plasma, 25.8 +/- 4.9% of PMN were retrained as compared with 11.1 +/- 2.7% suspended in zymosan-activated serum (ZAS) (P = 0.001) and 7.9 +/- 3.2% in fresh serum (P = 0.0005). Addition of heated plasma (56 degrees C for 30 min) to ZAS restored optimal retention. These results suggested a role for a heat-stable factor(s) in plasma, which augmented complement-mediated enhancement of PMN adhesiveness. This study was designed to determine whether fibrinogen enhanced retention by nylon columns of PMN in the presence of activated complement. Addition of defibrinated plasma to ZAS failed to enhance adhesiveness to nylon. The retention of PMN suspended in ZAS was 11.1 +/- 2.7%, as compared with 10.5 +/- 3.7% in ZAS plus defibrinated plasma. Retention of PMN suspended in ZAS plus fibrinogen was 27.92 +/- 13.2% as opposed to 16.35 +/- 7.5% (P = 0.045) in ZAs. In contrast, retention of cells in serum was 12.96 +/- 7.5%. These results suggest that the process which enhances PMN adhesiveness involves the interaction of cells, activated complement, and fibrinogen.
当多形核白细胞（PMN）悬浮于酵母聚糖激活的血浆中时，其被尼龙柱保留的能力显著增强。在酵母聚糖激活的血浆中，25.8±4.9%的PMN被保留，相比之下，悬浮于酵母聚糖激活血清（ZAS）中的PMN为11.1±2.7%（P = 0.001），在新鲜血清中的为7.9±3.2%（P = 0.0005）。向ZAS中添加加热血浆（56℃，30分钟）可恢复最佳保留效果。这些结果提示血浆中一种热稳定因子发挥了作用，其增强了补体介导的PMN黏附性增强作用。本研究旨在确定在激活补体存在的情况下，纤维蛋白原是否增强PMN被尼龙柱保留的能力。向ZAS中添加去纤维蛋白血浆未能增强对尼龙的黏附性。悬浮于ZAS中的PMN保留率为11.1±2.7%，而在ZAS加去纤维蛋白血浆中的为10.5±3.7%。悬浮于ZAS加纤维蛋白原中的PMN保留率为27.92±13.2%，而在ZAS中为16.35±7.5%（P = 0.045）。相比之下，细胞在血清中的保留率为12.96±7.5%。这些结果表明，增强PMN黏附性的过程涉及细胞、激活补体和纤维蛋白原之间的相互作用。
