Reddy A L, Fialkow P J
Int J Cancer. 1981;27(4):501-4. doi: 10.1002/ijc.2910270413.
We have shown previously, with X-chromosome linked phosphoglycerate kinase (PGK) cell markers, that the fibrosarcomas induced in mice by subcutaneous injection of 0.2-2.0 mg of methylcholanthrene (MCA) dissolved in olive oil had a multicellular origin. In this study we used a constant dose of MCA injected subcutaneously and compared the effects on tumorigenesis of using benzene or olive oil as the solvents. The benzene and olive oil groups differed in tumor incidence (100% versus 50%), location (muscle versus skin), and type (rhabdomyosarcomas versus fibrosarcomas). Each of the nine olive-oil MCA-induced tumors showed both PGK enzyme types, whereas 13 ot 16 tumors induced by MCA in benzene displayed only a single PGK type. This difference in PGK phenotypes suggests that the MCA-benzene neoplasms arose from one or a few cells. We conclude from these results that the nature of the solvent plays an important role in the type and number of cells from which tumors induced by subcutaneous injection of MCA arise.
我们之前利用X染色体连锁磷酸甘油酸激酶（PGK）细胞标记物表明，通过皮下注射溶解于橄榄油中的0.2 - 2.0毫克甲基胆蒽（MCA）在小鼠体内诱导产生的纤维肉瘤具有多细胞起源。在本研究中，我们皮下注射恒定剂量的MCA，并比较了使用苯或橄榄油作为溶剂对肿瘤发生的影响。苯组和橄榄油组在肿瘤发生率（100%对50%）、位置（肌肉对皮肤）和类型（横纹肌肉瘤对纤维肉瘤）方面存在差异。九个由橄榄油-MCA诱导的肿瘤中的每一个都显示出两种PGK酶类型，而在苯中由MCA诱导的16个肿瘤中的13个仅显示出单一的PGK类型。PGK表型的这种差异表明，MCA-苯肿瘤起源于一个或少数几个细胞。我们从这些结果得出结论，溶剂的性质在皮下注射MCA诱导产生的肿瘤所起源的细胞类型和数量方面起着重要作用。
