Margler L W, Mah R A
J Assoc Off Anal Chem. 1981 Jul;64(4):1017-20.
A method is presented for determining methyl mercury that combines thin layer chromatography and atomic absorption spectrophotometry. The procedure is based on a previously reported method and entails isolating methyl mercury on a thin layer chromatogram, scraping adsorbent into an ignition tube, heating the tube, and moving the mercury vapor under vacuum into an absorption cell where it absorbs light at 253.7 nm. The procedure is sensitive to 0.7 ng Hg. Methyl mercury is extracted from biological samples by distilling, and then extracting with dithizone-chloroform. The extract is concentrated and applied to a thin layer chromatographic plate. The overall sensitivity of the method varies with sample size. For 100 g samples, the method is sensitive to about 8 ng Hg (as methyl mercury)/g (9 ppb).
本文介绍了一种结合薄层色谱法和原子吸收分光光度法测定甲基汞的方法。该程序基于先前报道的方法，包括在薄层色谱图上分离甲基汞，将吸附剂刮入点火管，加热该管，并在真空下将汞蒸气转移到吸收池中，在那里汞蒸气吸收253.7nm的光。该程序对0.7ng汞敏感。通过蒸馏从生物样品中提取甲基汞，然后用双硫腙-氯仿萃取。提取物浓缩后应用于薄层色谱板。该方法的总体灵敏度随样品量而变化。对于100g样品，该方法对约8ng汞（以甲基汞计）/g（9ppb）敏感。
