Goshgarian H G, Rafols J A
J Comp Neurol. 1981 Sep 20;201(3):441-56. doi: 10.1002/cne.902010309.
The phrenic nucleus of the adult albino rat was studied by utilizing the O-dianisidine method for the retrograde transport of horseradish peroxidase in conjunction with the zinc chromate modification of the Golgi technique. Application of HRP to the transected phrenic nerve in the neck labeled a column of phrenic motor neurons from C3 to C5 in the ipsilateral spinal cord. However, when HRP was applied to the phrenic nerve intrathoracically, labeled neurons were found from C3 to C6. The long axis of the column of phrenic neurons was oriented tangentially from rostral to caudal poles. There was a gradual shift of the column from posterior to anterior and from lateral to medial positions in the ventral horn. The peroxidase material was also used to localize impregnated phrenic motor neurons in the Golgi sections and to provide quantitative data on phrenic motor neurons. In Golgi-impregnated material two types of phrenic neurons were distinguished on the basis of dendritic morphology and orientation. These neurons were designated (1) large neurons with smooth, radially oriented dendrites, and (2) smaller neurons with varicose, tangentially oriented dendrites. Both types of neurons had a small number of spines and bulbous appendages issuing from the dendritic trunks and branches. The dendritic fields of adjacent phrenic neurons overlapped extensively with one another and with dendrites of more distally placed ventral horn motor neurons. In peroxidase-labeled sagittal sections the dendrites of phrenic neurons were primarily oriented in the rostrocaudal plane. The mean total number of peroxidase-labeled neurons in the phrenic nucleus was 415.75 +/- 18.36 cells. In sagittal sections the mean long axis diameter of phrenic cell bodies was 34.5 micrometers. In frontal sections the mean long axis diameter of phrenic cell bodies was 22.5 micrometers. Thus, from direct measurement, the phrenic neurons were 34% longer in the sagittal plane than in the frontal plane. In the present study each phrenic nucleus contributed fibers only to the ipsilateral phrenic nerve, and no evidence for peripheral crossing of fibers was found.
利用邻联茴香胺法进行辣根过氧化物酶逆行运输，并结合高尔基技术的铬酸锌改良法，对成年白化大鼠的膈神经核进行了研究。将辣根过氧化物酶应用于颈部横断的膈神经，标记了同侧脊髓中从C3到C5的一列膈运动神经元。然而，当将辣根过氧化物酶应用于胸段膈神经时，发现标记的神经元从C3到C6。膈神经元柱的长轴从吻端到尾端呈切线方向排列。该柱在腹角从后向前、从外侧向内侧位置逐渐移位。过氧化物酶物质还用于在高尔基切片中定位浸染的膈运动神经元，并提供膈运动神经元的定量数据。在高尔基浸染材料中，根据树突形态和方向区分出两种类型的膈神经元。这些神经元被命名为：(1) 具有光滑、径向排列树突的大神经元，以及 (2) 具有曲张、切线方向排列树突的较小神经元。两种类型的神经元都有少量从树突主干和分支发出的棘和球状附属物。相邻膈神经元的树突场相互广泛重叠，并与更靠远端的腹角运动神经元的树突重叠。在过氧化物酶标记的矢状切片中，膈神经元的树突主要沿头尾平面排列。膈神经核中过氧化物酶标记神经元的平均总数为415.75±18.36个细胞。在矢状切片中，膈细胞体的平均长轴直径为34.5微米。在额状切片中，膈细胞体的平均长轴直径为22.5微米。因此，通过直接测量，膈神经元在矢状平面上比在额状平面上长34%。在本研究中，每个膈神经核仅向同侧膈神经贡献纤维，未发现纤维外周交叉的证据。
