Todd-Kulikowski H D, Parsons R G
J Immunol Methods. 1981;44(3):333-41. doi: 10.1016/0022-1759(81)90051-x.
A solid-phase radioimmunoassay system to quantitate human fibronectin has been developed. This assay utilizes 125I-labeled affinity purified IgG directed against human plasma fibronectin. The sensitivity of this system is comparable to conventional (labeled antigen) radioimmunoassays having a detection limit of approximately 0.5 ng. This assay is relatively rapid (less than 24 h), the reagents are stable at 4 degrees C (less than 2 months), and the reproducibility is excellent. Both human plasma and cellular fibronectin react equivalently in this assay.
已开发出一种用于定量人纤连蛋白的固相放射免疫分析系统。该分析方法利用针对人血浆纤连蛋白的125I标记亲和纯化IgG。该系统的灵敏度与传统（标记抗原）放射免疫分析相当，检测限约为0.5 ng。该分析相对快速（少于24小时），试剂在4℃下稳定（少于2个月），且重现性极佳。人血浆和细胞纤连蛋白在该分析中反应相同。
