Warnick G R, Albers J J, Bachorik P S, Turner J D, Garcia C, Breckinridge C, Kuba K, McNeely S, Hillerman G, King P, Muesing R, Most B, Lippel K
J Lipid Res. 1981 Aug;22(6):1015-20.
High density lipoprotein (HDL) can be quantitated by measurement of cholesterol in supernates after precipitation of low and very low density lipoprotein (LDL and VLDL) with heparin and Mn(2+). Supernatant turbidity, often observed with hypertriglyceridemic specimens, indicates incomplete sedimentation of LDL/VLDL and precludes accurate quantitation of HDL. Ten Lipid Research Clinic Laboratories compared an ultrafiltration technique for clearing turbid heparin-Mn(2+) supernates to current methods involving repeat precipitation of either the original specimen after dilution or the d > 1.006 g/ml fraction after removal of VLDL from the initial specimen by ultracentrifugation. Results for ultrafiltration of 429 turbid supernates averaged only slightly higher (1.0-1.1 mg/dl) than results by the dilution or ultracentrifugation methods on the same specimens, but this difference was found to be significant (P < 0.005). The agreement of the ultrafiltration method with the other two methods is indicated by the following linear regression equations: a), ultrafiltration = (0.964 x ultracentrifugation) + 2.4 mg/dl, and correlation coefficient = 0.926; and b), ultrafiltration = (0.936 x dilution) + 3.3 mg/dl, and correlation coefficient = 0.933. We conclude that ultrafiltration of turbid heparin-Mn(2+) supernates is a convenient alternative to precipitation after either dilution or removal of VLDL.-Warnick, G. R., J. J. Albers, P. Bachorik, J. Turner, C. Garcia, C. Breckinridge, K. Kuba, S. McNeely, G. Hillerman, P. King, R. Muesing, B. Most, and K. Lippel. Multi-laboratory evaluation of an ultrafiltration procedure for high density lipoprotein cholesterol quantification in turbid heparin-manganese supernates.
高密度脂蛋白（HDL）可通过用肝素和锰离子（Mn(2+)）沉淀低密度脂蛋白（LDL）和极低密度脂蛋白（VLDL）后，测量上清液中的胆固醇来进行定量。高甘油三酯血症标本常出现上清液浑浊，这表明LDL/VLDL沉淀不完全，无法准确测定HDL。十个脂质研究诊所实验室比较了一种用于清除浑浊肝素 - 锰离子上清液的超滤技术与当前的方法，当前方法包括对稀释后的原始标本或通过超速离心从初始标本中去除VLDL后d > 1.006 g/ml部分进行重复沉淀。对429份浑浊上清液进行超滤的结果平均仅比相同标本采用稀释或超速离心法的结果略高（1.0 - 1.1 mg/dl），但发现这种差异具有统计学意义（P < 0.005）。超滤法与其他两种方法的一致性由以下线性回归方程表示：a），超滤 = （0.964×超速离心） + 2.4 mg/dl，相关系数 = 0.926；b），超滤 = （0.936×稀释） + 3.3 mg/dl，相关系数 = 0.933。我们得出结论，对浑浊肝素 - 锰离子上清液进行超滤是稀释或去除VLDL后沉淀法的一种便捷替代方法。 - 沃尼克，G.R.，J.J.阿尔伯斯，P.巴乔里克，J.特纳，C.加西亚，C.布雷金里奇，K.库巴，S.麦克尼利，G.希勒曼，P.金，R.缪辛，B.莫斯特，和K.利佩尔。用于浑浊肝素 - 锰上清液中高密度脂蛋白胆固醇定量的超滤程序的多实验室评估。
