Albers J J, Warnick G R, Wiebe D, King P, Steiner P, Smith L, Breckenridge C, Chow A, Kuba K, Weidman S, Arnett H, Wood P, Shlagenhaft A
Clin Chem. 1978 Jun;24(6):853-6.
Plasma high-density lipoprotein is commonly estimated by measuring the cholesterol remaining in plasma supernatant solutions after other lipoproteins, which contain apolipoprotein B, are precipitated with heparin and Mn2+. The method (method I) now in use by the Lipid Research Clinics, in which Mn2+ is at 46 mmol/liter final concentration, is reasonably accurate, but precipitation and sedimentation of lipoproteins other than high-density lipoproteins is often incomplete. We evaluated two modifications of method I. In method II, the Mn2+ concentration was doubled; the second modification (method III) included the increased Mn2+ concentration in a combined heparin Mn2+ reagent, decreased sample volume (2 ml), and a shorter incubation time (10 min at room temperature). The percentages of samples with turbid supernates (i.e., incomplete sedimentation) by methods I, II, and III were 9, 3, and 2%, respectively. Among non-turbid supernates, the percentages of samples containing measurable apolipoprotein B (incomplete precipitation) were 79, 19, and 16%, respectively. We conclude that method III is the most convenient and accurate of the three procedures.
血浆高密度脂蛋白通常通过测量在其他含有载脂蛋白B的脂蛋白用肝素和Mn2+沉淀后血浆上清液中剩余的胆固醇来估算。脂质研究诊所目前使用的方法（方法I）中，Mn2+的最终浓度为46 mmol/升，该方法相当准确，但除高密度脂蛋白外的其他脂蛋白的沉淀和沉降往往不完全。我们评估了方法I的两种改进方法。在方法II中，Mn2+浓度加倍；第二种改进方法（方法III）包括在肝素-Mn2+联合试剂中提高Mn2+浓度、减少样品体积（2 ml）以及缩短孵育时间（室温下10分钟）。方法I、II和III产生浑浊上清液（即沉降不完全）的样品百分比分别为9%、3%和2%。在非浑浊上清液中，含有可测量载脂蛋白B（沉淀不完全）的样品百分比分别为79%、19%和16%。我们得出结论，在这三种方法中，方法III是最方便、最准确的。
